Protein Registration for Hit Identification

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General Information Collaborator () Collaborator Institution SGC Contact ()

Target Background

Please provide short descriptions of the following (if known):

Protein (sub-)family* Protein domain description* Biological function and disease link Known small molecule ligands for the target protein

Construct Information Vector Backbone* Full, purified construct AA sequence including tags*

Protein domain sequence *

UniProt ID

Protein Name Start End

Tags *

Select at least one tag per position, or both tags on one position.

N-term Tag His Biotin

C-term Tag His Biotin

Modifications (e.g., protein PTMs, isotope) Comments

Production Information Expression host * E. coli Insect cells Mammalian Yeast Other (please specify)

Purification protocol * (Briefly describe the purification steps) SEC Chromatogram *

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SDS-PAGE *

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Estimated purity (%) * %

Protein Storage Buffer * Buffer requirements for Stability (e.g. cofactors if relevant)

Mass Concentration (mg/mL) * Molar Concentration (μM) *

Aliquots * (recommended to be no greater than 100μL, preferably 50μL)

Volume (μL) * Number *

Oligomerisation/aggregation properties

Characterisation Data/QC SDS-PAGE of the purified sample *

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Stability Data

1. Freeze/Thaw – 1x thaw

Time 0h: Sample after thawing

Mass Spec Image *

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Measured Mw (Da) *

Time 2h: After thaw and 2h incubation at room temperature

Mass Spec Image *

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Measured Mw (Da) *

Size Exclusion Chromatogram *

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2. DMSO Tolerance – Thermal Shift Assay

Values of Tm and ΔTm *

Upload duplicate values from the DMSO tolerance assay

Tm curves *

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ΔTm curves *

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Comments

Any additional information you would like to disclose

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