Protein Registration for Hit Identification

This is for viewing purposes and does not save responses

required *
General Information () ()
Target Background

Please provide short descriptions of the following (if known):

Construct Information

A His-tag is required, on either terminus. Biotin is optional.

His Biotin
His Biotin
Production Information E. coli Insect cells Mammalian Yeast Other (please specify) (Briefly describe the purification steps)

Drag & drop image here or

Drag & drop image here or

% (must be >90% soluble)
(HEPES buffers preferred for storage. Please include any special additives for stability (e.g. cofactors))
Minimum 1 mg/mL / 30 μM; the higher of the two is the cutoff, depending on protein size.
At least 12 aliquots, all identical in volume, in 1.5 mL tubes; at least 120 nmol in total.

The total amount is calculated automatically as the aliquots are entered.

Characterisation Data/QC
Perform all Characterization (QC) experiments in the following buffer:
20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM TCEP

Drag & drop image here or

Stability Data

1. Freeze/Thaw – 1x thaw

Time 2h: After thaw and 2h incubation at room temperature

Drag & drop image here or

Drag & drop image here or

2. DMSO Tolerance – Thermal Shift Assay

Thermal Shift Assay in the presence of DMSO; DSF, nanoDSF, DSLS or related method that yields melting temperature in the presence of 0, 1, 3, and 5 % DMSO

Upload duplicate values from the DMSO tolerance assay

Drag & drop image here or

Drag & drop image here or

Any additional information you would like to disclose

Suggested Screening Assay Buffer

In addition to running screening and hit validation experiments with your protein, SGC would also like to be able to share your protein with partners in order to facilitate the creation of research reagents that will help the scientific community study the protein – e.g., research antibodies targeting the protein. If you are willing to allow SGC to share your protein for this purpose, please click the following box to give us your consent.

I give SGC permission to share my protein and information with partners for reagent generation, e.g. antibody reagents
By checking this box, you agree to be bound by and to become a party to the SGC's click-wrap protein submission terms and conditions (the "Terms"). Please carefully read the Terms and ensure that you have the proper authority to submit the protein to SGC in accordance with the Terms.