PROTEIN INTAKE FOR TARGET 2035

Thank you for your interest in providing protein to SGC for ligand screening to help us achieve the aims of Target 2035.

The purpose of this Intake Form is to help us determine whether your protein may be suitable for screening in one of our research programs.

• Review timeline: SGC will attempt to review your submitted Intake Form and respond within 7 days.
• Next steps: If your protein appears suitable, we may invite you to submit a Full Submission Form, which will request additional information and data about your protein.
• Terms: Submitting an Intake Form carries no obligations. However, submitting a Full Submission Form will require agreement to SGC’s click-wrap protein submission terms and conditions, which can be reviewed here.

ESSENTIAL REQUIREMENTS BEFORE SUBMISSION

• Protein amount: 120 nmol (equals to 6 mg of a 50 kDa protein)
• Purity: >90% soluble, no aggregation
• Tags:
  Mandatory: His-Tag (on either terminus)
  Preferred: Biotin-labeled (either through Avi-tag or another Tag/treatment)
  Optional: Protease cleavage sites
  Prohibited: No Solubility tags!
• Sample Homogeneity (single purification)

ALIQUOT REQUIREMENTS

MANDATORY QUALITY CONTROL DATA REQUIRED FOR FULL SUBMISSION

If your protein is accepted, you will need to provide:

• Purification data (final SDS-PAGE gel, storage buffer, etc.)
• Size Exclusion and Mass Spectrometry profiles after 1 freeze/thaw cycles and 2 hours incubation at room temperature
• Thermal Shift Assay in the presence of DMSO; DSF, nanoDSF, DSLS or related method that yields melting temperature in the presence of 0, 1, 3, and 5 % DMSO

* Indicates required question

Request Information

Full Name * Your Title / Role * Your Institution / Employer * Your e-mail address * SGC Contact SGC Contact e-mail address Protein Name * (max 100 characters) UniProt ID * (max 100 characters) Amino Acid Sequence* (provide full construct sequence including tags)

Biotinylated (preferred)

His-tag*

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