PAP39 (PRPSAP1)

PDB:2C4K

Revision

Revision Type:created
Revised by:created
Revision Date:created
Entry Clone Accession:gi|14290488
Entry Clone Source:MGC
SGC Clone Accession:
Tag:N-terminal hexahistidine tag with integrated TEV protease cleavage site: mhhhhhhssgvdlgtenlyfq*s(m).
Host:BL21(DE3)-Gold containing the chaperon plasmid pG-Tf2 (Takara).

Construct

Prelude:
Sequence:

MHHHHHHSSGVDLGTENLYFQSMRTGYRVFSANSTAACTELAKRITERLGAELGKSVVYQETNGETRVEIKESVRGQDIFIIQTIPRDVNTAVMELLIMAYALKTACARNIIGVIPYFPYSKQSKMRKRGSIVCKLLASMLAKAGLTHIITMDLHQKEIQGFFSFPVDNLRASPFLLQYIQEEIPNYRNAVIVAKSPDAAKRAQSYAERLRLGLAVIHGEAQCTELDMDDGRHSPPMVKNATVHPGLELPLMMAKEKPPITVVGDVGGRIAIIVDDIIDDVESFVAAAEILKERGAYKIYVMATHGILSAEAPRLIEESSVDEVVVTNTVPHEVQKLQCPKIKTVDISLILSEAIRRIHNGESMAYLFRN

Vector:pNIC-Bsa4

Growth

Medium:
Antibiotics:
Procedure:

Purification

Procedure

Extraction

Procedure

Concentration:
Ligand
MassSpec:
Crystallization:
NMR Spectroscopy:
Data Collection:
Data Processing: