MT

PDB:2C2N

Revision

Revision Type:created
Revised by:created
Revision Date:created
Entry Clone Accession:MTA -s003
Entry Clone Source:MGC
SGC Clone Accession:
Tag:
Host:E.coli BL21DE3 R3

Construct

Prelude:
Sequence:

Vector:pNIC28-Bsa4

Growth

Medium:
Antibiotics:
Procedure:Medium: TB + 34 µg/ml Chloramp. Cells were grown in 1 liter TB in 2.5-L baffled flasks, which was inoculated with 10 ml overnight culture. The culture was grown at 37°C to OD=2.3, and transferred to 25°C. 1 mM IPTG was then added, and incubation continued for 4 hours. The cells were then collected by centrifugation and frozen at -80°C.

Purification

Procedure
Column 1: Ni-affinity, HisTrap, 1 ml (GE/Amersham)

Extraction

Procedure

Concentration:
Ligand
MassSpec:
Crystallization:Column 1: Ni-affinity, HisTrap, 1 ml (GE/Amersham)
NMR Spectroscopy:
Data Collection:
Data Processing: