SPR Assay Data



All data were generated by the Biophysics team at the Structural Genomics Consortium, University of Toronto and are part of SGC initiatives including CACHE (lead: Matthieu Schapira), ASMS screening (lead: Hui Peng, Levon Halabelian) and DEL screening (lead: Rafael Counago). Interface developed by Lihua Liu (Schapira group).
Screening Platform
HTS Stage


Compound ID
(Toronto internal ID or ChemiReg ID)
(seperated by semicolon, comma or space)
Compound Common Name
(seperated by semicolon, comma or space)
Target Name
Experiments Performed :
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Info Criteria:
  • KD < 100 µM
  • %Binding (Affinity) between 30 and 200
  • %Affinity Chi² / Rmax between 0 and 15
  • Acceptance state is not Rejected
  • Max tested concentration > 1.5 x KD
Row Number Assay Type Screening Platform SPR Chip Type HTS stage ChemiReg ID Common Name SMILES Structure Supplier Purification Sample ID Target ID Buffer Temperature (℃) Instrument Affinity Model Kinetics Model Protein Immobilization Conditions Run Conditions Immobilized Protein Level (RU) Immobilized Protein MW (Da) Top Compound Concentration (μM) Min concentration (μM) Max concentration (μM) Adjusted relativeAnalyte binding Relative (RU)C-O binding Relative (RU)% Rmax (%)Binder prediction Certainty (%)ClassificationBinding to reference (%)Baseline difference (RU)Association slope (%)Dissociation slope (%)Dissociation respons (%)ExcludedCut-offSlopeSlow dissociationR > RmaxAcceptance stateCurve markersKD (M)T(KD)RmaxT(Rmax)offset (RU)Rmax (Theor) (RU)%Binding (Affinity) (%)%Binding (Kinetic) (%)Acceptance certainty (%)KD (fitted Rmax)/KD (constant Rmax)Affinity Chi² (RU²)Kinetics Chi² (RU²)ka (1/Ms)(kinetics)T(ka)(kinetics)kd (1/s)(kinetics)T(KD)(kinetics)Rmax (RU)(kinetics)T(Rmax)(kinetics)KD (M)(kinetics)tc(kinetics)U-value(kinetics)Drift (RU/s)(kinetics)KD (M)(constant)T(KD)(constant)Rmax (RU)(constant)T(Rmax)(constant)offset (RU)(constant)Plots Dose Response image fileSensorgrams Dose Response image fileSensorgrams single concentration image file Experiments were performed by Experiment Date Comments